HROC450Met1 T0 M1

Categories: Intestine cancer cell lines Organism: Human Tissue: Metastasis Disease: Colorectal Adenocarcinoma Metastatic Site: Liver Age: 59 years Gender: Male Growth Properties: Adherent Citation: HROC450Met1 T0 M1 (Cytion catalog number 300725) Biosafety Level: 1 Msi Status: MSS Culture Medium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: TrypLE Express 15 min 37°C Subculturing: Seeding after thawing 4*104/cm2