HCC4006

Description: HCC4006 is a human non-small cell lung cancer (NSCLC) cell line derived from a lung adenocarcinoma. It is characterized by an activating exon 19 deletion in the EGFR gene, which makes it particularly sensitive to EGFR tyrosine kinase inhibitors (TKIs) such as erlotinib and gefitinib. This feature has made HCC4006 a widely used model for studying EGFR-mutant NSCLC and resistance mechanisms to EGFR-targeted therapies. In the Cancer Cell Line Encyclopedia (CCLE), HCC4006 has been comprehensively profiled at the genomic, transcriptomic, and epigenetic levels, confirming its high sensitivity to EGFR inhibition and highlighting its use as a pharmacogenomic reference model. High-resolution genomic studies have revealed that HCC4006 displays a relatively simple karyotype compared to other NSCLC models, which may facilitate clearer interpretation of drug responses and genomic alterations. It lacks common resistance mutations such as T790M in the EGFR gene, making it suitable for modeling initial treatment responses. However, resistance can be induced in vitro, allowing researchers to study mechanisms of acquired resistance. For example, resistance to EGFR TKIs in HCC4006 has been linked to epithelial-mesenchymal transition (EMT) and activation of alternative signaling pathways, such as AXL kinase overexpression. HCC4006 has also been assessed in large-scale transcriptomic comparisons of cell lines and primary tumors. It is one of the lung adenocarcinoma cell lines that demonstrates a moderate correlation to primary tumor gene expression profiles, though the degree of correlation can vary depending on the purity of the tumor samples used for comparison. These analyses underscore the relevance of HCC4006 in modeling certain molecular aspects of lung adenocarcinoma, particularly those associated with EGFR-driven oncogenesis, while also emphasizing its limitations in fully recapitulating the heterogeneity of primary tumors. Organism: Human Tissue: Metastatic Disease: Lung adenocarcinoma Metastatic Site: Pleural effusion Synonyms: HCC-4006, Hamon Cancer Center 4006 Age: >50 years Gender: Male Ethnicity: Caucasian Morphology: epithelial Cell Type: epithelial cell Growth Properties: Adherent Citation: HCC4006 (Cytion catalog number 305785) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_1269 Mutational Profile: Mutation: EGFR, Simple, p.Leu747_Glu749del (c.2239_2247delTAAGAGAA), Heterozygous (ATCC=CRL-2871, TP53, Simple, p.Tyr205His (c.613T>C), Homozygous (DepMap=ACH-000066). Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: Accutase Doubling Time: 46 hours Fluid Renewal: 2 to 3 times per week Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Disclaimer: Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Certificate Of Analysis: The certificate of analysis can be requested on the website or via email at info@cytion.com. Please indicate the lot number of your product in the email. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 3: 860015.0 Required Product 4: 830100.0