Anti-CD19 CAR-Gamma Delta T Cells (delta2)

Anti-CD19 CAR-Gamma Delta T Cells (delta2) are produced by high-titer lentiviral transduction of human peripheral blood gamma delta 2 (delta2) T cells using the Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3zeta, SIN Vector, #78601). These ready-to use CAR (chimeric antigen receptor)-Gamma Delta T cells express an anti-CD19 CAR consisting of the ScFv (Single chain fragment variable) of anti-CD19 (clone FMC63) linked to a 2nd generation CAR containing the CD8 hinge and transmembrane domains, and the 4-1BB and CD3zeta signaling domains (Figure 1). These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays. Figure 1: Construct diagram showing components of the anti-CD19 CAR expressed in Anti-CD19 CAR- Gamma Delta T Cells (delta2).