Firefly Luciferase-Nuclear eGFP Lentivirus

The Firefly Luciferase-Nuclear eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain firefly luciferase and nuclear eGFP (Luc2-P2A-nuclear eGFP) driven by an EF1A promoter. These lentiviruses also transduce a puromycin selection marker (Figure 1). The nuclear eGFP has NLS sequences at the C-terminus. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.