Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-CD28-CD3zeta, IL-15/IL-15Ra)

The Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-CD28-CD3zeta, IL-15/IL-15Ra) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv portion of anti-CD19 (clone FMC63) linked to a 4th generation CAR (Chimeric Antigen Receptor) containing the CD8 hinge and transmembrane domains, CD28 and CD3zeta signaling domains. This construct also includes a 2A-IL15/IL-15 receptor alpha (IL15Ra) sequence downstream of the anti-CD19 CAR cassette to create armored CAR-T cells by overexpressing IL-I5 and IL-15Ra (Figure 1). Note: This product transduces the same construct as the anti-CD19 CAR Lentiviruses (CD19 ScFv-CD8-4-1BB-CD3zeta, IL-15/IL-15Ra) (#82378), but differs in a key aspect: 82379, described here, is constructed with a CD28 costimulatory domain in the anti-CD19 CAR cassette, while 82738 contains a 4-1BB costimulatory domain. Cat # Self-Inactivation (SIN) Selection Marker Armored with Costimulatory domain 82379 yes no IL-15/IL-15Ra CD28 82378 yes no IL-15/IL-15Ra 41BB 78775 yes eGFP N/A 41BB 78602 yes puromycin N/A 41BB 78601 yes no N/A 41BB 78600 no puromycin N/A 41BB Figure 1. Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-CD28-CD3zeta, IL-15/IL-15Ra) construct diagrams.(A) Schematic of the lenti-vector used to generate the anti-CD19 CAR lentivirus. The vector is a SIN vector, and it contains the IL15/IL-15 receptor alpha (IL15Ra) sequence downstream of the anti-CD19 CAR cassette. (B) Construct diagram showing components of the anti-CD19 CAR