WM983B Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM983B. WM983B is a human metastatic melanoma cell line that was established from an inguinal node metastatic site in a 54-year-old male patient. The subject displayed malignant melanoma of polypoid type, level IV with a tumor thickness of 2.5 cm. MW983B line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM983B is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B BR, and WM983C. WM983B cells produce xenograft tumors when injected into immunocompromised mice.