WM3918 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3918. WM3918 is a metastatic human melanoma cell line. These cells need 5-10% of FBS to adhere to culture flask. This cell line expresses wild type BRAF, N-RAS, c-KIT, and CDK4 genes. WM3918 cells produce xenograft tumors when injected into immunocompromised mice.