WM3854 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3854. WM3854 is a human metastatic melanoma cell line that displays a mesenchymal morphology. This cell line was established from a heavily pigmented metastatic site (right upper flank of back) in a 64-year-old male with superficial and deep inguinal nodes forming a 3.0 cm mass. This cell line contains a Q61L mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is known to be wild type for BRAF, c-KIT and CDK4. WM3854 cells produce xenograft tumors when injected into immunocompromised mice.