WM3772F Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM3772. WM3772F is a primary human metastatic uveal melanoma cell line displaying clumpy pigmented morphology. These cells require 5% FBS to adhere to culture flask. This cell line was established from a metastases arising from uveal melanoma of a patient. Uveal melanoma is a rare type of cancer of an eye and can arise in the anterior (iris) or the posterior (ciliary body or choroid) uveal tract. Most uveal tract melanomas originate in the choroid. The ciliary body is less commonly a site of origin, and the iris is the least common. This cell line expresses wild type BRAF, N-RAS, c-KIT, and CDK4 genes. WM3772F cells match with WM3618F. These cells produce xenograft tumors when injected into immunocompromised mice.