WM3743 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3743. WM3743 is a primary post chemotherapy melanoma cell line that displays a mesenchymal morphology. This cell line expresses wild type N-RAS, c-KIT and CDK4 genes. WM3743 cells produce xenograft tumors when injected into immunocompromised mice.