WM373 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM373. WM373 is a metastatic human melanoma cell line that displays a pigmented morphology and grows in tight adherent dense colonies. This cell line was established from a left upper back metastasis site in a 49-year-old male with level IV superficial spreading melanoma. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. WM373 cells produce xenograft tumors when injected into immunocompromised mice.