WM3702 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3702. WM3702 is a metastatic human melanoma cell line that displays pigmented, thin elongated morphology in culture. This cell line was established from a right upper flank of back in a 58-year-old male with necrotic fungating melanoma. The subject displayed metastatic melanoma forming multiple subcutaneous nodules with overlying skin ulcerations. This cell line contains a Q61L mutation at position 61 in the N-RAS gene. The Q61L mutation results in an amino acid substitution at position 61 in NRAS, from a glutamine (Q) to a leucine (L). WM3702 cells match with WM3702 and WM3682. These cells produce xenograft tumors when injected into immunocompromised mice.