WM3630 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3630. WM3630 is a metastatic human melanoma cell line that displays a mesenchymal and fibroblastic morphology and form tight networks in culture. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses C70T (Cys70Thr) mutation in CDK4 gene and is wild type for N-RAS and c-KIT. WM3630 cells produce xenograft tumors when injected into immunocompromised mice.