WM3629 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3629. WM3629 is a metastatic human melanoma cell line that displays a mesenchymal and dendritic morphology. This cell line was established from a lymph node metastasis site of a patient. This cell line contains a D549G mutation in the BRAF gene resulting in an amino acid substitution from aspartic acid to glycine at codon 549 (Asp549Gly). These cells also express G12D mutation in the N-RAS gene resulting in substitution of glycine with aspartic acid at codon 12 (Gly12Asp).WM3629 cells produce xenograft tumors when injected into immunocompromised mice.