WM266-4 Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM266-4. WM266-4 is a metastatic human melanoma cell line with small flat mesenchymal morphology. This cell line was derived from the same patient as the cell lines WM115, WM293A, and WM165-1. WM115 cell line originated from the primary tumor, and WM266-4, WM165-1 and WM293A were from individual lymph-node metastases. The subject (55-year-old female) displayed VGP with a Clark level III tumor with thickness of 2.24mm.This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion. WM266-4 cell line is wild type for N-RAS, c-KIT, and CDK4 genes. WM266-4 cells produce xenograft tumors when injected into immunocompromised mice.