WM2044 Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM2044. WM2044 is a metastatic human melanoma cell line that displays mesenchymal morphology. These cells require insulin for growth in culture. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion, and is wild type for N-RAS, c-KIT, and CDK4. WM2044 cells produce xenograft tumors when injected into immunocompromised mice.