WM1791C Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM1791C. WM1791C is a metastatic human melanoma cell line. This cell line features the wild type KRAS and KRAST501, KRASE62K mutation. This cell line also expresses a K22Q mutation in CDK4. The K22Q mutation results in an amino acid substitution at position 22 in CDK4, from a lysine (K) to a glutamine (Q). This cell line is wild type for BRAF, and c-KIT genes. WM1791C cells produce xenograft tumors when injected into immunocompromised mice.