WM1361A Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM1361A. WM1361A is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. These cells display mesenchymal morphology in culture. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. This cell line also expresses PTEN loss of function including hemizygous PTEN deletion and is wild type for BRAF, c-KIT, and CDK4. WM1361A cells produce xenograft tumors when injected into immunocompromised mice.