LbCas12a mRNA V2 (Cap1, m1Psi)

Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. The LbCpf1/Cas12a allow efficient mutagenesis in zebrafish and Xenopus (Moreno-Mateos, et al. Nat Communications, 2017, 8: 2024). LbCpf1 increases homology-directed genome editing. This mRNA features: - A Cap 1 structure with high capping efficiency - Codon optimized using GenScript X5 algorithm to enhance expression efficiency - 100% substitution with N1-methyl-pseudouridine (m1Psi) for improved protein expression and reduced innate immune response - GenScript's patented UTR to enhance translation - A 100A poly(A) tail to mimic natural mature mRNA. Concentration: 1mg/mL. Full mRNA length: 4063 nt. Full mRNA Molecular Weight: 1.32x10^6 Da. Storage buffer: 1mM Sodium citrate, pH 6.5. OD260/OD280: 1.70 ~ 2.30. Capping Efficiency: >= 90%.