Firefly Luciferase saRNA (Cap-1)

Firefly luciferase (FLuc) self-amplifying RNA (saRNA) (Cap-1) encodes firefly luciferase, a protein originally isolated from the firefly P. pyralis, that catalyzes the ATP-dependent oxidation of the molecule luciferin (D-luciferin, Cay25836, Cay14682, Cay14681) resulting in chemiluminescence at a wavelength of approximately 560 nm. It is capped with the Cap-1 structure to increase translation efficiency. FLuc saRNA (Cap-1) is based on the self-replicating Venezuelan equine encephalitis virus (VEEV), which results in stable expression of FLuc. It can be used as a control for the delivery and translation efficiency of RNA. Encapsulation of FLuc saRNA in lipid nanoparticles (LNPs) has been used for saRNA delivery and expression of FLuc in animal models.. SMILES: . InChi Code: . InChi Key: