EGFP IVT mRNA (m2psi substitution)

The enhanced form of the green fluorescent protein, which was initially obtained from the jellyfish Aequorea Victoria, will be expressed by the EGFP mRNA. In mammalian cell culture, EGFP is a frequently employed direct detection reporter that produces brilliant green fluorescence with an emission peak at 509 nm. Croyez's EGFP mRNAs was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.