Tissue, Total Protein, Human Tumor, Melanoma HisTek(TM)

Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. Applications:  Electrophoresis, Western Blot and Immunoprecipitation,  Enzymatic Activity,  Protein-DNA Interaction,  Protein-Protein Interaction,  Tissue-Specific Expression Identification, Quality Control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot., 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.