Tissue, Membrane Protein, Human Adult Normal, Epididymis HisTek(TM)

Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. Applications: Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interaction, Tissue-Specific Expression Identification. Other applications not tested. Recommended Dilutions: Western Blot: 10-20ug/lane , Optimal dilutions to be determined by the researcher. Quality Control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot., 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.