Vasoactive Intestinal Peptide (VIP) BioAssay(TM) ELISA Kit (Human)

The Human Vasoactive Intestinal Peptide (VIP) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of VIP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. Detection Range: 6.17-500pg/ml, Sensitivity: 2.34pg/ml, Precision: Intra-Assay CV: <10%, Inter-Assay CV: <12%, Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for VIP has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled VIP and unlabeled VIP (standards or samples) with the pre-coated antibody specific for VIP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of VIP in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of VIP in the sample. Kit Components: *028858A: Microtiter Plate, 96 wells, Pre-coated, ready to use, *028858B: Standard, 2x1vial , 028858C: Standard Diluent, 1x20ml, *028858D: Detection Reagent A, 1x120ul , *028858E: Detection Reagent B, 1x120ul , 028858F: Assay Diluent A, 1x12ml, 028858G: Assay Diluent B, 1x12ml, 028858H: TMB Substrate, 1x9ml, 028858K: Stop Solution, 1x6ml, 028858L: Wash Buffer, 30X, 1x20ml, Storage and Stability: Store *028858A, *028858B, *028858D and *028858E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.