Trefoil Factor 3, Intestinal (TFF3) BioAssay(TM) ELISA Kit (Mouse)

Trefoil Factor 3, Intestinal (TFF3) BioAssayT ELISA Kit (Mouse) is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement of mouse TFF3 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.125ng/ml, Intra-Assay: CV<10%, Inter-Assay: CV<12%, Kit Components: *028606A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use, *028606B: Standard, 2x1vial , 028606C: Standard Diluent, 1x20ml, *028606D: Detection Reagent A, 1x120ul , *028606E: Detection Reagent B, 1x120ul , 028606F: Assay Diluent A, 1x12ml, 028606G: Assay Diluent B, 1x12ml, 028606H: TMB Substrate, 1x9ml, 028606K: Stop Solution, 1x6ml, 028606L: Wash Buffer, 30X, 1x20ml, Storage and Stability: Store *028606A, *028606B, *028606D and *028606E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.