TNFRSF18/GITR BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse TNFRSF18/GITR in cell culture supernates, serum and plasma (heparin, EDTA). Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. , Assay Range:, 62.5pg/ml-4000pg/ml, Sensitivity: < 10 pg/ml , Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: O35714, Entrez Gene ID: 21936, Kit Components: 96-well plate pre-coated with anti-TNFRSF18/GITR, Mouse, Lyophilized recombinant TNFRSF18/GITR, Mouse standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.