Sheep PRL(Prolactin) ELISA Kit

Prolactin acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation Assay Type: Sandwich. Sensitivity: 0.25 ng/mL. Standard: 50 ng/mL. Detection range: 0.79-50 ng/mL. Sample type: serum, plasma and other biological fluids. Assay length: 3.5h. Research Field: Endocrinology,Reproductive science,Genetic science,Hormone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep PRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep PRL in the samples is then determined by comparing the OD of the samples to the standard curve.