Sex Hormone Binding Globulin, Human (SHBG) BioAssay(TM) ELISA Kit

Sex Hormone Binding Globulin, Human (SHBG) BioAssay(TM) ELISA Kit
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
028063.96 96 tests - -

3 - 19 Werktage*

941,00 €
 
The Human Sex Hormone Binding Globulin (SHBG) ELISA Kit is a sandwich enzyme immunoassay for in... mehr
Produktinformationen "Sex Hormone Binding Globulin, Human (SHBG) BioAssay(TM) ELISA Kit"
The Human Sex Hormone Binding Globulin (SHBG) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SHBG in human serum, plasma and other biological fluids. Detection Range: 62.5-4,000pg/ml, Sensitivity: 22.9pg/ml, Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to human SHBG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for human SHBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SHBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SHBG in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *028063A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use, *028063B: Standard, 2x1vial , 028063C: Standard Diluent, 1x20ml, *028063D: Detection Reagent A (green), 1x120ul , *028063E: Detection Reagent B (red), 1x120ul , 028063F: Assay Diluent A, 2x, 1x6ml, 028063G: Assay Diluent B, 2x, 1x6ml, 028063H: TMB Substrate, 1x9ml, 028063K: Stop Solution, 1x6ml, 028063L: Wash Buffer, 30X, 1x20ml , Storage and Stability: Store *028063A, *028063B, *028063D and *028063E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.
Schlagworte: ABP, SBP, SHBG, TeBG, Sex steroid-binding protein, Sex hormone-binding globulin, Testosterone-estrogen-binding globulin, Testosterone-estradiol-binding globulin, Testis-specific androgen-binding protein
Hersteller: United States Biological
Hersteller-Nr: 028063

Eigenschaften

Anwendung: ELISA
Spezies-Reaktivität: human
Format: Solid Phase

Datenbank Information

UniProt ID : P04278 | Passende Produkte
Gene ID GeneID 6462 | Passende Produkte

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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