Rat NKB(Neurokinin B) ELISA Kit

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Is a critical central regulator of gonadal function (By similarity) Assay Type: Competitive Inhibition. Sensitivity: 4.12 pg/mL. Standard: 1000 pg/mL. Detection range: 15.63-1000 pg/mL. Sample type: Serum, plasma and other biological fluids.. Assay length: 2.5h. Research Field: Neuro science,Hormone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NKB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NKB in the samples is then determined by comparing the OD of the samples to the standard curve.