Rat LAMP2 (Lysosomal Associated Membrane Protein 2) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LAMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LAMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LAMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LAMP2 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 62.5-4000 pg/mL. Sensitivity: 26.7 pg/mL. Assay Type: Sandwich. Standard: 4000 pg/mL. Sample Type: sSerum, plasma and other biological fluids. Assay duration: 3.5h. Protein Function: Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy (PubMed:11082038, PubMed:8662539). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (By similarity). Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:11082038, PubMed:8662539). Functions by binding target proteins, such as GAPDH, NLRP3 and MLLT11, and targeting them for lysosomal degradation (PubMed:11082038, PubMed:18644871, PubMed:8662539). In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2 (PubMed:18644871). Plays a role in lysosomal protein degradation in response to starvation (PubMed:11082038). Required for the fusion of autophagosomes with lysosomes during autophagy (By similarity). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (By similarity). Required for normal degradation of the contents of autophagosomes (By similarity). Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation, antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits (By similarity). Is not required for efficient MHC class II-mediated presentation of endogenous antigens (By similarity) [The Uniprot Consortium]