Rat IL-1alpha ELISA Kit

Interleukin-1alpha (IL-1alpha) and interleukin-1beta (IL-1beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1alpha gene is assigned to chromosome 2. Genetic polymorphisms at IL-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1alpha and IL-1beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18kDa. ScienCell's rat IL-1alpha ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IL-1alpha specific-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IL-1alpha captured in strips.