Rat FTH (Ferritin, Heavy Polypeptide) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FTH in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Stores iron in a soluble, non-toxic, readily available form (By similarity). Important for iron homeostasis (By similarity). Has ferroxidase activity (By similarity). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (By similarity). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (By similarity) [The Uniprot Consortium]