Pig CRP(C Reactive Protein) ELISA Kit

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity) Assay Type: Sandwich. Sensitivity: 0.112 ng/mL. Standard: 500 ng/mL. Detection range: 7.81-500 ng/mL. Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.. Assay length: 3.5h. Research Field: Tumor immunity,Infection immunity,Cardiovascular biology,Rheumatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CRP in the samples is then determined by comparing the OD of the samples to the standard curve.