Mouse IL-23 ELISA Kit

Interleukin-23 subunit alpha (IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis, and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. The International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812). ScienCell's mouse IL-23 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL-23-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection monoclonal antibodies (clone No. 35928.11) are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse IL-23 in the sample that is captured on the strips.