Mouse CA2 (Carbonic Anhydrase II) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CA2 in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Catalyzes the reversible hydration of carbon dioxide (By similarity). Can also hydrate cyanamide to urea (By similarity). Involved in the regulation of fluid secretion into the anterior chamber of the eye (By similarity). Essential for bone resorption and osteoclast differentiation (By similarity). Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption (By similarity). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (By similarity) [The Uniprot Consortium]