Monoclonal Anti-cGMP EIA Kit without Acetylation [1x96 well]

Based on the unique mouse monoclonal Anti-cGMP antibody. It is the only EIA kit on the market with monoclonal anti-cGMP antibody (all other kits are based on polyclonal anti-cGMP antibodies). No more hazardous chemical (acetic anhydride ) for your cGMP assay. The monoclonal Anti-cGMP antibody has similar affinities for non-acetylated cGMP and acetylated cGMP. Thus acetylation with the acetic anhydride is permanently eliminated from the assay. Super sensitivity and selectivity. The monoclonal Anti-cGMP antibody displays >108 fold of selectivity over cAMP, GTP, and other nucleoside analogues. The Anti-cGMP EIA Kit provides significantly improved sensitivity and selectivity over other kits based on polyclonal anti-cGMP antibodies on the market. Shortest assay time and high-throughput format. The Anti-cGMP EIA Kit is the best choice for drug screenings. NewEast Biosciences cGMP ELISA Kit is a competitive immunoassay to measure the cGMP levels, either from cell extracts or from in vitro guanylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cGMP in cell extracts or in in vitro guanylyl cyclase assays will competitively bind to the monoclonal anti-cGMP antibody in the presence of fixed amounts of cGMP-conjugated horse-radish peroxidase. Known amounts of cGMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm. The intensity of the yellow color is inversely proportional to the concentration of cGMP in samples. The measured optical density is used to calculate the concentration of cGMP in samples based on the curve from the cGMP standards.