Monoclonal Anti-cAMP EIA Kit without Acetylation [96 well]

Based on the unique mouse monoclonal Anti-cAMP antibody. It is the only EIA kit on the market with monoclonal anti-cAMP antibody (all other kits are based on polyclonal anti-cAMP antibodies). No more hazardous chemical (acetic anhydride ) for your cAMP assay. The monoclonal Anti-cAMP antibody has similar affinities for non-acetylated cAMP and acetylated cAMP. Thus acetylation with the acetic anhydride is permanently eliminated from the assay. Super sensitivity and selectivity. The monoclonal Anti-cAMP antibody displays >108 fold of selectivity over cGMP, ATP, and other nucleoside analogues. The Anti-cAMP EIA Kit provides significantly improved sensitivity and selectivity over other kits based on polyclonal anti-cAMP antibodies on the market. Shortest assay time and high-throughput format. The Anti-cAMP EIA Kit is the best choice for drug screenings. NewEast Biosciences cGMP ELISA Kit is a competitive immunoassay to measure the cGMP levels, either from cell extracts or from in vitro guanylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cGMP in cell extracts or in in vitro guanylyl cyclase assays will competitively bind to the monoclonal anti-cGMP antibody in the presence of fixed amounts of cGMP-conjugated horse-radish peroxidase. Known amounts of cGMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm. The intensity of the yellow color is inversely proportional to the concentration of cGMP in samples. The measured optical density is used to calculate the concentration of cGMP in samples based on the curve from the cGMP standards.