IL-23R BioAssay(TM) ELISA Kit (Mouse)

Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand- dependent manner. Sample Type: Cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Mouse IL-23R. Sensitivity: <10pg/ml, Range: 78pg/ml-5000pg/ml, Specificity: Recognizes natural and recombinant Mouse IL-23R. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Mouse IL-23R BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A a monoclonal antibody from rat specific for IL-23R has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: I25-I373) and test samples are added to the wells, a biotinylated detection a biotinylated polyclonal antibody from goat specific for IL-23R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-23R amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Mouse Il23r antibody, 1x plate, Lyophilized recombinant Mouse Il23r standard, 2x10ng/tube, Biotinylated anti-Mouse Il23r antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.