IGFBP-1 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IGFBP-1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25kD. , Assay Range:, 31.2pg/ml-2000pg/ml, Sensitivity: < 1 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P08833, Entrez Gene ID: 3484, Kit Components: 96-well plate pre-coated with anti-IGFBP-1, Human, Lyophilized recombinant IGFBP-1, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.