Human TMPO (Thymopoietin) ELISA (Small Sample Volume)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMPO in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1 , TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide [The Uniprot Consortium]