Human NLRC5 (Protein NLRC5) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NLRC5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NLRC5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NLRC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NLRC5 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 31.3-2000 pg/mL. Sensitivity: 18.3 pg/mL. Assay Type: Sandwich. Standard: 2000 pg/mL. Sample Type: sSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.. Assay duration: 3.5h. Protein Function: Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms [The Uniprot Consortium]