Human MGP (Matrix Gla Protein) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MGP in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation [The Uniprot Consortium]