Human MASP2 (Mannose Associated Serine Protease 2) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MASP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MASP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MASP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MASP2 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 1.56-100 ng/mL. Sensitivity: 0.63 ng/mL. Assay Type: Sandwich. Standard: 100 ng/mL. Sample Type: sserum, plasma and other biological fluids. Assay duration: 3.5h. Protein Function: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase [The Uniprot Consortium]