Human KPNA4 (Karyopherin subunit alpha-4) ELISA Kit (HUFI03671)

Human KPNA4 (Karyopherin subunit alpha-4) ELISA Kit from Assay Genie is a pre-coated immunoassay with a high sensitivity and broad range and has been designed to measure Human KPNA4 (Karyopherin subunit alpha-4) in serum, plasma & cell culture supernatant samples. The Human KPNA4 (Karyopherin subunit alpha-4) ELISA Kit accurately measures natural Human KPNA4 (Karyopherin subunit alpha-4) levels quantified versus standard curves obtained and is based on antibodies raised against a recombinant Human KPNA4 (Karyopherin subunit alpha-4) peptide. Protein function: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. [The UniProt Consortium]