Human IL4R (Interleukin 4 Receptor) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL4R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL4R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL4R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL4R in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 31.25-2000 pg/mL. Sensitivity: 7.92 pg/mL. Assay Type: Sandwich. Standard: 2000 pg/mL. Sample Type: sSerum, plasma, tissue homogenates and other biological fluids. Assay duration: 3.5h. Protein Function: Receptor for both interleukin 4 and interleukin 13 (PubMed:17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2 , Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells [The Uniprot Consortium]