Human IL-1alpha ELISA Kit

Interleukin-1-a (IL-1a) and interleukin-1-beta (IL-1beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1a has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1a is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1a gene is assigned to chromosome 2. Genetic polymorphisms at IL-1a and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1a and IL-1beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1a with the molecular mass of 18kDa. ScienCell's human IL-1a ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IL-1a-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IL-1a captured in strips.