Human HBb (Hemoglobin Beta) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Involved in oxygen transport from the lung to the various peripheral tissues , LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure , Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation [The Uniprot Consortium]