Human CSN1 (Casein Alpha) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN1 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 1.57-100 ng/mL. Sensitivity: 0.53 ng/mL. Assay Type: Sandwich. Standard: 100 ng/mL. Sample Type: stissue homogenates, cell lysates, cell culture supernates, breast milk and other biological fluids. Assay duration: 3.5h. Protein Function: Important role in the capacity of milk to transport calcium phosphate , Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors [The Uniprot Consortium]