Human CEA ELISA Kit

Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180kDa, comprised of 60% of carbohydrates. It is found in adenocarcinomas of endodermally-derived digestive system epithelia and in fetal colon. CEA is one of the most widely used tumor markers in serum immunoassays in carcinomas. ScienCell's human CEA ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CEA-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human CEA in the sample that is captured on the strips.