ELISpot Human IL-8 (CXCL8), pre-coated (5 plates)

The Human IL-8 (CXCL8) Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IL-8 secretion by stimulated effector cells. Pre-coated kits are ready-to-use, helping labs save time and reduce human touchpoints during setup, ideal for accelerating research workflows. Interleukin-8 (IL-8 / CXCL8) is a pro-inflammatory chemokine that plays a key role in the recruitment and activation of immune cells, particularly neutrophils. It is secreted by multiple cell types, including monocytes, macrophages, epithelial cells, and endothelial cells, in response to inflammatory stimuli or microbial products. IL-8 is best known for directing neutrophil chemotaxis to sites of infection or tissue injury and enhancing their activation and degranulation. In addition to its role in innate immunity, IL-8 can also act on subsets of T cells, particularly memory and activated T cells that express CXCR1 or CXCR2 promoting their migration into inflamed tissues and contributing to the shaping of local immune responses. Due to its role in immune cell recruitment, inflammation, and tissue remodeling, IL-8 is widely used as a biomarker in studies of infection, inflammation, and tumor immunology. Protein function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. [The UniProt Consortium]